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. 2006 Sep 18;26(22):8599–8606. doi: 10.1128/MCB.01062-06

FIG. 2.

FIG. 2.

Decreased binding of CREB to the mutant CRE in the bcl-2 promoter. (A) Diagram of the bcl-2 5′ region with the location of the CRE and Sp1 sites at the P1 promoter region. The Cdx and TATA sites are shown at the P2 promoter. (B) Quantitative ChIP assays for the binding of CREB, ATF-2, and CBP at the bcl-2 promoter in B cells from wild-type (Wt) and mutCRE-bcl2 (mutCRE) mice. The results are displayed as the percentage of input DNA. The primers were specific for the region of the bcl-2 promoter with the CRE and Sp1 sites. (C) Quantitative ChIP assays for the binding of NF-κB transcription factors p50, p52, p65, Rel-B, and c-Rel, and Sp1 to the bcl-2 promoter in B cells from wild-type and mutCRE-bcl2 mice. The primers used were the same as those used for panel B.