Skip to main content
. 2006 Sep 18;26(22):8599–8606. doi: 10.1128/MCB.01062-06

FIG. 3.

FIG. 3.

Decreased expression of bcl-2 in B cells from mutCRE-bcl2 mice. (A) Real-time RT-PCR analysis of bcl-2 mRNA in splenic B cells from wild-type (Wt) and mutCRE-bcl2 mice. GAPDH was used as the control for normalization, and the level of bcl-2 mRNA in wild-type B cells was designated as 1. (B) Western blot analysis of Bcl-2 protein in splenic B cells from wild-type and mutCRE-bcl2 mice at 8 weeks of age. Expression of Bcl-2 is normalized to the expression of GAPDH. (C) Western blot analysis of Bcl-2 protein in splenic B cells from wild-type and mutCRE-bcl2 mice at 13 months of age. Expression of Bcl-2 is normalized to the expression of GAPDH. (D) Real-time RT-PCR of bcl-2 mRNA in heart, liver, brain, lung, kidney, thymus, and spleen from wild-type and mutCRE-bcl2 mice. GAPDH was the control for normalization, and the level of bcl-2 mRNA in heart from mutCRE-bcl2 mice was defined as 1.