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Bcl-2 levels in activated B cells from wild-type (Wt) and mutCRE-bcl2 mice. Purified splenic B cells from wild-type and mutCRE-bcl2 mice were left untreated (control) or were treated with 20 ng/ml PMA plus 0.5 μg/ml ionomycin (P + I), 30 μg/ml anti-IgM, or 15 μg/ml LPS for 30 h. The immunoblots were probed for Bcl-2 and β-actin, and the level of Bcl-2 was normalized to that of β-actin.
