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. 2006 Sep 11;26(23):8814–8825. doi: 10.1128/MCB.00636-06

FIG. 3.

FIG. 3.

Targeting luciferase expressing reporter constructs to PML NBs alters expression in a promoter-dependent fashion. (A) SK-N-SH cells were transfected with 100 ng pGL3-TetO and increasing amounts of TetR-PML (white bars) or PMLIV (black bars). (B) SK-N-SH cells were transfected with 100 ng biotinylated (white bars) or unbiotinylated (black bars) pGL3 plasmid and increasing amounts of BBP-PMLIV plasmid as indicated. (C) SK-N-SH cells were transfected with increasing amounts of pGL3-LacO and either 10 ng LacI-SpT DNA (dotted line) or no targeting vector as a control (solid line). (D) To ensure that LacI-SpT did not induce repression alone, cells were transfected with 100 ng pGL3-LacO with no SpT, 100 ng pGL3 with 10 ng LacI-SpT, or 100 ng pGL3-LacO with 10 ng LacI-SpT. (E) SK-N-SH cells were transfected with 100 ng biotinylated (white bars) or unbiotinylated (black bars) CMV-pGL3 with increasing amounts of BBP-PMLIV as indicated. *, P was <0.05; **, P was <0.01. Error bars represent the standard deviations of three separate measurements.