Skip to main content
. 2006 Sep 11;26(23):8814–8825. doi: 10.1128/MCB.00636-06

FIG. 6.

FIG. 6.

Targeting plasmid DNA to PML NBs enhances the transcriptional potential of ICP0. (A) Cells were transfected with 100 ng pGL3-TetO and TetR-PMLIV (black bars) or PMLIV (white bars) as well as increasing amounts of ICP0 as indicated. Luciferase activity was normalized to the well containing no ICP0. **, P was <0.01. (B) Immunofluorescence images of double labeling using anti-PML and anti-ICP0 antibodies. Top panels show an image for visualization of the presence of PML NBs (imaged with a 100× objective). Bottom panels images show both transfected and untransfected cells. (imaged with a 40× objective). (C) Cells were transfected with 100 ng pGL3-TetO and TetR-PMLIV (black bars) or PMLIV (white bars) as well as increasing amounts of E4orf3 as indicated. Luciferase activity was normalized to the well containing no E4orf3. (D) Cells transfected with E4orf3 and PML (top panels) relative to cells transfected with only E4orf3. Error bars represent standard deviations. Bar = 5 μm.