FIG. 2.

Strand-specific repair of CPDs in the DHFR gene. (A) DNA prepared as for Fig. 1 was digested with KpnI and then with T4 endonuclease V and subjected to Southern analysis with RNA probes specific for the transcribed (filled symbols) or nontranscribed (open symbols) strand of the constitutively expressed DHFR gene. Dotted lines, naive cells; solid lines, cells differentiated with TPA for 48 h. Data are averages of two experiments, and error bars are standard errors of the means. (B) Same data as those for panel A combined with those in Fig. 1. Repair of the nontranscribed strand in differentiated cells was replotted after subtraction of the amount of GGR observed in Fig. 1. Upward triangles, monocytes; downward triangles, THP1; leftward triangles, HL60. Error bars were omitted for clarity.