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. 2006 Sep 25;26(23):8880–8891. doi: 10.1128/MCB.00751-06

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Copyright © 2006, American Society for Microbiology

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FIG. 3. — A p53-dependent checkpoint is activated in S6-heterozygous embryos. (A) Immunohistochemistry of sections of E6.5 S6^wt^/^wt and S6^wt^/^del embryos with antibodies against p53. (B) Morphology of representative E7.5 S6^wt^/^wt/p53⁻^/⁻, S6^wt^/^del/p53⁻^/⁻, and S6^wt^/^del embryos. (C) Percentage of mitotic cells in the embryonic region of E7.5 S6^wt^/^wt/p53⁻^/⁻ (n = 8), S6^wt^/^del/p53⁻^/⁻ (n = 8), and S6^wt^/^del (n = 7) embryos. (D) Percentage of apoptotic cells in E7.5 S6^wt^/^wt/p53⁻^/⁻ (n = 8), S6^wt^/^del/p53⁻^/⁻ (n = 7), and S6^wt^/^del (n = 8) embryos. S6^wt^/^wt/p53⁻^/⁻, S6^wt^/^del/p53⁻^/⁻, and S6^wt^/^del genotypes in C and D are indicated with 1, 2, and 3, respectively. (E) Whole-mount preparations of representative E8.5 and E9.5 S6^wt^/^wt/p53⁻^/⁻ and S6^wt^/^del/p53⁻^/⁻ embryos. (F) Immunohistochemistry of sections of E6.5 S6^wt^/^wt and S6^wt^/^del embryos with antibodies against the ATM/ATR phosphorylated consensus sequence (p-S/TQ) and phosphohistone H2A.X (γ-H2A.X). The majority of cells from E6.5 S6^wt^/^wt embryos stained strongly positive with these antibodies 30 min after gamma irradiation (5Gy). (G) Morphology of representative S6^wt^/^wt/p19Arf ⁻^/⁻ (n = 14) and S6^wt^/^del/p19Arf ⁻^/⁻ (n = 11) embryos at E8.5. (H) Morphology of representative S6^wt^/^wt/p21⁻^/⁻ and S6^wt^/^del/p21⁻^/⁻ embryos at E8.5 and E9.5. Scale bar, 50 μm (A and F). Error bars in panels C and D denote SD. Embryos in panels B, E, G, and H were genotyped by PCR analysis.