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. 2006 Sep 25;26(23):8857–8867. doi: 10.1128/MCB.01031-06

FIG. 3.

FIG. 3.

LZTS2 represses β-catenin-mediated transcription. (A) One hundred nanograms of pGL3-OT (OT) or pGL3-OF (OF), 25 ng of pcDNA3-β-gal, 5 ng of TCF1 expression vector, 20 ng of β-catenin, and various amounts of pcDNA3-FLAG-hLZTS2 as indicated were transfected into LNCaP cells. Cells were cultured for 24 h in the regular media and luciferase, and β-Gal activities were measured as indicated above. Similar experiments were repeated with PC3 cells (B) and DU145 cells (C). (D) Either 100 ng of pGL3-OT (OT) or pGL3-OF (OF), 25 ng of pcDNA3-β-gal, and other shRNA or expression constructs as indicated in the figure were transfected into the human colon cancer cell line SW480. Luciferase and β-Gal activities were measured in panel C.