FIG. 6.

LZTS2 regulates the cellular level of β-catenin. The FLAG-tagged expression vectors containing wild-type APC (A) or LZTS2 (B) or the LZTS2 NES mutant (C) were transfected into SW480 cells. Cells were stained with FLAG and β-catenin antibodies for the ectopically expressed proteins and endogenous β-catenin, as indicated by arrows. (D) Percentages of positively stained β-catenin cells were measured from the above experiments. The intensity and localization of β-catenin staining in the nuclei (N), cytoplasm (C), or both (N and C) were analyzed in cells transfected with different expression constructs as indicated. (E) SW480 cells were infected with the pLentiviral vectors for either wild-type or mutated LZTS2. The nuclear extracts and whole-cell lysates were prepared from the above cells and used for Western blotting.