TABLE 1.
Absolute numbers of thymocytes in different mouse linesa
| Genotype (n) | Mean no. of thymocytes ± SD (106)
|
|||
|---|---|---|---|---|
| Total | Double negative (CD4− CD8−) | Double positive (CD4+ CD8+) | Single positive and ISP (CD4+ CD8−, CD4− CD8+) | |
| Littermate control (14) | 132 ± 33 | 6 ± 1.3 | 103 ± 29 | 15 ± 3.2 |
| lck-Cre/ShcWT (13) | 139 ± 27 | 8 ± 1.8 | 114 ± 24 | 15 ± 5 |
| lck-Cre/ShcF317 (5) | 140 ± 26 | 7 ± 1.9 | 115 ± 23 | 18 ± 2.6 |
| lck-Cre/ShcFF239/240 (9) | 61 ± 27 | 6 ± 1.1 | 48 ± 2 | 7 ± 2.7 |
| lck-Cre/ShcFFF (11) | 28 ± 7 | 6 ± 1.6 | 21 ± 4 | 4 ± 0.9 |
| lck-Cre/shcfl/fl (3) | 34 ± 7 | 8 ± 2.5 | 21 ± 5 | 4 ± 1.1 |
a
Absolute numbers of thymocytes from different transgenic and knockout mice are shown. The absolute numbers were obtained by multiplying the total thymocyte number by the percentages of DN, DP, SP, and intermediate single positive (ISP) populations. Note that the n values shown here are underrepresentations of the number of mice analyzed for each genotype, as only cell numbers from mice concurrently analyzed are indicated. However, essentially similar numbers of thymocytes and their subsets were obtained for other mice that were independently analyzed (e.g., >50 lck-Cre/ShcFF and lck-Cre/shcfl/fl mice have been analyzed to date).