FIG. 5.

Decreased pCaMKIV/Gr staining in brain subregions of fear-conditioned CaMKKα KO mice. (A) Representative sections (×4 magnification) through the hippocampus of WT mice and CaMKKα KO mice placed in the training box with no further manipulation (Basal) or subjected to tone-shock paired training. Positive staining is associated with deposition of a dark precipitate after peroxidase staining (black arrowheads). (B) Quantification of pCaMKIV/Gr immunohistochemistry. Densitometric analysis of sections of WT and KO mice is shown (three or four mice for each group) (ACC, anterior cingulated cortex; SSC, somatosensory cortex [layer IV]). The average density of the WT basal group is normalized to 1 for each brain region. Values that are significantly different from the WT tone-shock group are indicated by asterisks (*, P = 0.014; **, P = 0.02; ***, P = 0.001).