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. 2006 Aug 14;26(20):7539–7549. doi: 10.1128/MCB.00508-06

FIG. 2.

FIG. 2.

(A) Changes of pluripotency and differentiation marker gene expression in ES cells during sodium vanadate treatment. Total RNA was isolated from Nanog β-geo ES cells after being treated with 25 μM sodium vanadate for the indicated periods of time (hours) and was subjected to RT-PCR analysis. (B) Sodium vanadate treatment of ES cells with or without exogenous Nanog expression. TRE-Nanog ES cells contain an inducible Nanog transgene under a tet-off gene induction system (13). The cells were treated with 25 μM sodium vanadate in the presence or absence of doxycycline (1 μg/ml) (DOX) for 24 h and subjected to RT-PCR analysis. The expression of endogenous Nanog mRNA decreased upon sodium vanadate treatment regardless of Nanog transgene expression, whereas the expression of Zfp42 and Gata6 mRNA was not changed by the addition of sodium vanadate when the Nanog transgene was expressed. (C) Protein levels of Nanog, Oct3/4, and Sox2 after sodium vanadate treatment. Nanog β-geo ES cells were treated with sodium vanadate for 48 h at the indicated concentrations and subjected to Western blotting. Only the Nanog protein decreased within 48 h after treatment.