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. 2006 Aug 14;26(20):7539–7549. doi: 10.1128/MCB.00508-06

FIG. 3.

FIG. 3.

Grb2 deficiency abrogated the effects of sodium vanadate on Nanog repression. (A) Wild-type and Grb2 null ES cells were in suspension culture with or without sodium vanadate (50 μM) for 48 h. Live phase-contrast images were shown. Bars, 200 μm. (B) RT-PCR analysis of gene expression in wild-type and Grb2 null ES cells. Total RNA was prepared after 48 h of sodium vanadate treatment at the indicated concentrations. (C) RT-PCR analysis of gene expression in Grb2 null ES cells and Grb2 addback-Grb2 null ES cells treated with or without sodium vanadate (50 μM) for 48 h.