FIG. 4.

Inhibition of Mek blocked the effects of sodium vanadate on Nanog repression. (A) Western blotting using phosphospecific antibodies against Erk, Akt, or Jnk. Nanog β-geo ES cells were treated with sodium vanadate for 48 h at the indicated concentrations. (B) The effect of specific kinase inhibitors on sodium vanadate-induced Nanog repression. Western blotting was performed after 48 h of treatment with or without sodium vanadate (25 μM) and/or kinase inhibitors, as indicated. (C) RT-PCR analysis was performed after 48 h of treatment with or without sodium vanadate (25 μM) and/or kinase inhibitors, as indicated. DMSO, dimethyl sulfoxide.