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FGFR-dependent Mek activation repressed the Nanog gene during ES cell aggregation. Whole-mount X-Gal staining (blue) of Nanog β-geo ES cell aggregates treated with (A) dimethyl sulfoxide, (B) Mek inhibitor (PD98059; 25 μM), (C) PI3K inhibitor (LY294002; 20 μM), (D) Jnk inhibitor (SP600125; 20 μM), or (E) FGFR-specific inhibitor (SU5402; 20 μM). The paraffin sections were counterstained with eosin Y (pink). Bars, 100 μm.
