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. 2006 Aug 21;26(20):7358–7371. doi: 10.1128/MCB.00149-06

FIG. 1.

FIG. 1.

Deltex1 is a Notch regulator that is induced by Notch signaling in T cells. (A) Deltex1 mRNA is induced by CBF1/RBPJκ-dependent Notch signaling in T-cell lines. The Northern blot shows expression of Deltex1 mRNA in two thymoma cell lines (AKR1 and 1010) and in 3T3 fibroblasts overexpressing the intracellular domains of Notch1 (Notch1-IC), Notch3 (Notch3-IC), and Notch1-mM2-2 (Notch1-ICmut), which contains a mutation in the RAM domain. Northern blots were hybridized with a probe detecting the 3′ untranslated region of Deltex1, and ethidium bromide staining of the ribosomal RNAs is shown as a loading control. (B) TaqMan RT-PCR analysis of Deltex1, pre-Tα, Notch1, and Hes1 on cDNA prepared from 3T3 cells transduced with control or Notch1-IC retrovirus (as described for panel A) or thymocytes derived from transgenic Lck-Notch-IC mice (15) or littermate controls. (C) Overexpression of Deltex1 inhibits ligand-dependent Notch signaling. RT-PCR analysis shows induction of Deltex-1, pre-Talpha, Notch1, and Hes1 mRNA in 1010 (open bars) or 1010 overexpressing Deltex1 (filled bars) cultured on stromal cells expressing empty vector (OP9) or Delta1 (OP9-Delta1).