Skip to main content
. 2006 Aug 21;26(20):7791–7805. doi: 10.1128/MCB.00022-06

FIG. 5.

FIG. 5.

Treatments that disrupt CCP-mediated endocytosis inhibit the internalization of BMP receptors. COS7 or C2C12 cells were transfected with HA-BRIb or myc-BRII-LF as described for Fig. 3. In some experiments, the cells were cotransfected with a sixfold excess of dyn2-K44A along with the BMP receptor construct. Hypertonic treatment, cytosol acidification, and treatment with CP were employed as described in the text. (A) Typical images of the effects of hypertonic treatment (sucrose) or CP (100 μM, 15 min) on the endocytosis of HA-BRIb and myc-BRII-LF in COS7 cells. Similar levels of inhibition were observed following cytosol acidification (data not shown), as well as in cells cotransfected with dyn2-K44A and in C2C12 cells (see panel B). Bar, 20 μm. (B) Quantification by the point confocal method. The fluorescence intensity measured at time zero on similarly treated cells was taken as 100% for each specific treatment. Bars represent means ± SEM of measurements conducted on 80 to 140 cells in each case. A comparison of pairs on treated versus untreated cells demonstrates highly significant effects of all the treatments on both COS7 and C2C12 cells (P < 10−12 and in some cases P < 10−25). Analogous results (not shown) were obtained for BRIb in C2C12 cells.

HHS Vulnerability Disclosure