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. 2006 Aug 21;26(20):7791–7805. doi: 10.1128/MCB.00022-06

FIG. 7.

FIG. 7.

Effect of cav-1α, cav-1-siRNA, or wt dyn2 and -K44A expression on BMP signaling. (A) C2C12 cells were transfected with HA-tagged cav-1α and stimulated with 20 nM BMP-2 for 30 min. Western blot analyses using anti-P-Smad1/5-specific antibodies reveal no significant change in Smad1/5 phosphorylation. For a loading control, the same membrane was incubated with anti-β-actin antibody. (B) Cells were transiently transfected with cav-1α, BRII-LF, and BRIa as indicated, together with reporter constructs. cav-1α expression significantly reduced both ligand-independent and -dependent BMP signaling. Error bars represent standard deviations from three different assays. (C) cav-1-specific siRNA was transfected in C2C12 cells to knock down endogenous cav-1 expression. A P-Smad1/5 Western blot shows no influence of cav-1 downregulation on the phosphorylation level. (D) Expression of HA-tagged wt dyn2 and the dyn-K44A mutant shows no significant alteration in the level of phosphorylated Smad1/5. Controls confirm wt dyn2, dyn2-K44A, and β-actin expression. WB, Western blotting; w/o, without.

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