FIG. 1.

Expression of NFAT upon adipocyte differentiation, in obesity, and in Nfatc2^−/− Nfatc4^−/− mice. Expression of NFATc2 and NFATc4 were determined upon adipocyte differentiation using 3T3/L1 cells (A) and with a genetic model of obesity using ob/ob mice (B). Cell extracts from different stages of adipocyte differentiation were examined by immunoblotting analysis to assess the expression of NFAT and C/EBP members (A). The expression of PPARγ and β-actin is also indicated. Semiquantitative RT-PCR was performed to assess the expression level of NFAT messages in epididymal fat depots of 8-week-old control and ob/ob mice (B). Relative intensity of NFAT messages was normalized to the expression of GAPDH and presented (C). The level of NFAT members in epididymal fat depots of Nfatc2^−/− Nfatc4^−/− mice (DKO) and control Nfatc2^−/+ Nfatc4^−/+ mice was determined by RT-PCR (D) and immunoblotting analysis (E). The expression level of adipocyte markers (PPARγ, C/EBPα, and fatty acid binding protein aP2) in Nfatc2^−/− Nfatc4^−/− mice (DKO) or control Nfatc2^−/+ Nfatc4^−/+ mice was also shown (D and E).