FIG. 6.

Abundance of HSF is differentially increased at heat shock gene promoters upon temperature shift. (A) Abundance of HSF before heat shock at the indicated promoters. Real-time PCR signals for nonshocked samples were normalized to PHO5 and to input. (B) Change of HSF abundance at the HSP12 promoter during the time course of heat shock relative to the non-heat-shock level (0′), which was arbitrarily set at 1. (C and D) The same experiment as shown in panel B, except that signals were obtained for the HSP82 and SSA4 promoters, respectively.