TABLE 1.
Abundance of histones at indicated promoters before heat shock, relative to the PHO5 promoter
| Promoter | Strain expressing construct | Abundancea
|
|
|---|---|---|---|
| Total H3 | Acetylated H3 | ||
| HSP12 | WTb HSF | 1.1 ± 0.3 | 0.7 ± 0.1 |
| HSF(1-424) | 1.3 ± 0.2 | 0.6 ± 0.2 | |
| HSF(1-40Δ147-833) | 0.8 ± 0.2 | 0.9 ± 0.2 | |
| HSF(1-40Δ147-583) | 1.3 ± 0.2 | 0.9 ± 0.1 | |
| HSP82 | WT HSF | 0.8 ± 0.1 | 0.7 ± 0.1 |
| HSF(1-424) | 0.7 ± 0.2 | 0.6 ± 0.1 | |
| HSF(1-40Δ147-833) | 0.9 ± 0.1 | 1.1 ± 0.3 | |
| HSF(1-40Δ147-583) | 1.1 ± 0.1 | 0.8 ± 0.1 | |
| SSA4 | WT HSF | 0.8 ± 0.1 | 1.1 ± 0.1 |
| HSF(1-424) | 1.1 ± 0.2 | 1.1 ± 0.1 | |
| HSF(1-40Δ147-833) | 0.8 ± 0.2 | 1.1 ± 0.1 | |
| HSF(1-40Δ147-583) | 1.0 ± 0.1 | 1.2 ± 0.2 | |
a
Values (means ± standard deviations) were obtained by normalization of the real-time PCR signal of the specified promoter to the signal of the PHO5 promoter and to the input signal.
b
WT, wild type.