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. 2006 Aug 14;26(20):7451–7465. doi: 10.1128/MCB.00684-06

FIG. 2.

FIG. 2.

Recombination in hpr1-101 and hpr1-103 mutants. (A) The isogenic strains W303-1A (WT), WH101-1A (hpr1-101), WH103-1A (hpr1-103), and SChY58a (hpr1Δ), carrying the mutant alleles integrated in the chromosomal HPR1 locus, were transformed with plasmid pSCh204 (L-lacZ recombination assay) or pSCh206 (L-PHO5 recombination assay). Gray boxes represent LEU2 repeats that flank either the PHO5 or the lacZ ORF, as indicated. The white box represents the promoter, and the white solid arrow represents the CYC transcription termination site. Shown are recombination frequencies under repressed conditions (glucose) in cells transformed with plasmid pRS314GLlacZ, containing the GL-lacZ recombination assay, in which transcription is under the control of the GAL1 promoter. The recombination frequencies shown are averages of two to four different experiments. (B) Northern analysis of the L-lacZ and L-PHO5 recombination system in isogenic strains W303-1A (WT [wild type]), WH101-1A (hpr1-101), and SChY58a (hpr1Δ) transformed with either pSCh204 or pSCh206, respectively. Filters were hybridized with either a lacZ or a PHO5 probe and with the 25S rRNA probe. All data were normalized with respect to the rRNA signal. The averages of two experiments are plotted. A.U., arbitrary units.