TABLE 5.

Oligonucleotide repair of a directly resectable I-SceI DSB and of a hairpin-capped DSB

Genotype	I-SceI break-targeting oligonucleotide				IR break-targeting oligonucleotide
	e		f		w		c
	No. of transformants per 107 cellsa	Relative frequencyb	No. of transformants per 107 cells	Relative frequency	No. of transformants per 107 cells	Relative frequency	No. of transformants per 107 cells	Relative frequency
WT	93,606 (85,560-107,459)	1	129,847 (117,771-143,139)	1	636 (511-795)	1	1,281 (1,167-1,470)	1
mre11	28,709 (14,648-33,353)	0.3c	20,282 (13,776-21,752)	0.2c	0 (0-2)	0.0016	2 (2-5)	0.0016
rad50	26,100 (21,138-34,770)	0.3c	40,608 (35,772-50,883)	0.3c	0 (0-0)	<0.0016	0 (0-3)	0.00078
xrs2	54,577 (43,337-71,083)	0.6c	44,484 (35,542-56,787)	0.3c	2 (0-2)	0.003	4 (2-6)	0.003

^aMedian and >95% confidence interval, or range (in parentheses) when the number of repeated experiments was <6, of Trp⁺ transformant colonies per 10⁷ viable cells, resulting from targeting single-strand oligonucleotide e or f to the I-SceI break at the CORE-I-SceI cassette or oligonucleotide w or c to a spontaneous break associated with an Alu-IR. Survival was ∼30 to 40% after transformation with or without oligonucleotides for the wild type and all mutant strains tested.

^bTransformation frequency relative to the wild type (WT). The relative transformation frequency was calculated by dividing the absolute frequency for each mutant by the absolute frequency for the WT obtained with the corresponding oligonucleotide. All values have statistically significant decreases from the WT based on nonoverlapping 95% confidence intervals.

^cPlasmid transformation frequency is reduced about threefold in mre11, rad50, and xrs2 mutants. When these frequencies are normalized by plasmid transformation frequency, there is no statistically significant difference from that of the WT.