FIG. 6.

Manifestations of the ph^P1 trf2^P1 mutation. (A) The mutant phenotype of the ph^P1 trf2^P1 flies (scallops on the posterior wing margin and arista to tarsus transformation) and the results of rescue experiments with the P{trf2_175} and P{trf2_75} constructs. (B) Wild-type and ph^P1 trf2^P1 stage 11 and 12 embryos stained with antibodies against Vnd specific for the ventral nervous system. Expanded area of ventral neuroblasts in the region of segments A2 to A4 is observed. The borders of abnormal parasegments with a phenotype similar to that caused by mutations in segment polarity genes are indicated by a dashed line. (C) Staining of the wild-type and the ph^P1 trf2^P1 stage 15 embryos with antibodies against Engrailed reveals the irregular size of parasegments and wide strips of Engrailed expression (shown by arrow). (D) Staining of stage 16 embryos with 22C10 antibodies (DSHB, Iowa) that are specific for PNS shows significant abnormalities in ventral and lateral clusters of PNS compared with the wild type. Arrows indicate the ventral and lateral clusters in one parasegment. All embryos are shown anterior left and ventral down.