FIG. 7.
TRF2 expression in ovaries of wild-type and mutated females. (A and D) The confocal image of germarium (Ge) and developing cysts from ovarioles of wild-type (A) and mutated phP1 trf2P1 females (D) stained for TRF2 (green) and DNA (red). The nuclei of terminal filament cells (tfc), cap cell (cc), germ stem cells (gsc), inner sheath cells (isc), follicular cells (fc), nurse cells (nc), and the region of mitotically dividing cystocytes (cys) are indicated. In panels A to E, arrowheads show the nuclei of oocytes. (B) Magnified oocyte nucleus from panel A demonstrating colocalization of TRF2 and DNA. (C) The confocal image of stage 5 cysts stained for TBP (green) and DNA (red). (E) The confocal image of stage 5 cysts from phP1 trf2P1 females stained for TRF2 and DNA. (F) Magnified oocyte nucleus from panel C. In all preparations, DNA was stained with propidium iodide, Ab1 was used for TRF2, and polyclonal rabbit antibodies were used for TBP. (G to J) Defects in egg chamber development caused by the trf2 mutation revealed by DAPI staining. (G) Wild-type ovarioles of a 3-day-old female show properly organized cysts. (H) Mutant ovaries of a 3-day-old female; joined cysts are shown by arrowheads, and an arrow indicates the perishing cyst. (I) Two cysts covered by the same monolayer of follicular cells (arrowhead). (J) Cysts containing an abnormal number of nuclei of different sizes (arrowhead). Bar, 25 μm.