FIG. 7.

TReP-132 silencing prevents the induction of differentiation by progesterone. Synchronized T47D cells transfected with control or TReP-132 or siRNA (700 ng) and treated with progesterone (30 nM) or vehicle were harvested after 12 h (A) or 6 days (B and C) for quantitative RT-PCR analyses (A and B) and Oil Red O staining of lipid droplets (C). In panels A and B, mRNA levels are expressed relative to vehicle-incubated control siRNA-transfected cells, which were arbitrarily set as 1. NS, not significant; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 versus control. C. Lipid detection. Left panel: representative Oil Red O-stained cellular sections. Right panel: quantification of the lipid staining intensity.