FIG. 8.

Cdc20 promotes the degradation of phosphorylated wild-type AML1 but not nonphosphorylated AML1. 293T cells were cotransfected with FLAG-tagged wild-type AML1 and either empty vector, Cdh1 expression vector, or Cdc20 expression vector, as indicated above the lanes. At 48 h after transfection, FLAG-AML1 was immunoprecipitated, and the immunoprecipitate was used for Western blotting with anti-serine 303-phosphorylated AML1 antibodies and anti-AML1 antibodies. Western blotting was also performed with samples of lysate, and anti-HA antibodies were used to detect Chd1 and Cdc20 expression.