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. 2006 Aug 14;26(20):7409–7419. doi: 10.1128/MCB.00585-06

FIG. 2.

FIG. 2.

Identification and quantitation of PRC protein levels. (A) PRC protein was identified in 293FT cell extracts by using antibodies raised to three different subregions [anti-PRC(95-533), anti-PRC(1047-1379), and anti-PRC(400-467)] of the molecule. Extracts were prepared from cells transfected with either empty vector (pSV-Sport) or vector expressing the full PRC open reading frame (PRC/pSV-Sport). (B) Total BALB/3T3 or U2OS cell extracts were prepared from proliferating cells, serum-starved cells, or starved cells stimulated with serum for either 3 or 8 h. In both panels, proteins were detected following denaturing gel electrophoresis and immunoblotting with either the indicated antibodies (A) or anti-PRC(1047-1379) (B).