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. Author manuscript; available in PMC: 2006 Nov 16.
Published in final edited form as: Immunol Rev. 2006 Oct;213:36–47. doi: 10.1111/j.1600-065X.2006.00436.x

Fig. 9. Immunofluorescent staining for syntaxin-4, an intracellular transport protein (SNARE), in mononuclear phagocyte-like THP-1 cells.

Fig. 9

This SNARE is present in the monocyte cell line THP-1 following differentiation with phorbol myristate acetate for 24 h and activation with IFNγ for an additional 24 h. Preliminary experiments suggest that proteins such as syntaxin-4 are altered in these cells following treatment with rHLA-G5, which could affect secretion of cytokines. Blue signal, DAPI nuclear stain; green signal, anti-syntaxin-4. The insert shows negative results with the isotype control for anti-syntaxin-4. Original magnifications ×250.