Table 3.
Cell composition of the bronchoalveolar lavage fluid and enzyme markers of lung inflammation from CF and wild-type (WT) mice at the indicated time points after a single dose of P aeruginosa LPS with and without pre-treatment with azithromycin (10 mg/kg/day), by oral administration, for 4 weeks.
| Alveolar Macrophages × 104/ml | Neutrophils × 104/ml | MPO OD × 10-3/second | LDH U/L | |||
| CF | LPS 3 h | AZM- | 7.9 ± 0.6 | 0.6 ± 0.1 | 21.2 ± 4.2 | 50.0 ± 11.5 |
| AZM+ | 3.6 ± 0.7† | 0.4 ± 0.2 | 30.1 ± 4.7 | 52.6 ± 17.6 | ||
| LPS 24 h | AZM- | 6.4 ± 0.9 | 130.9 ± 7.9 | 51.7 ± 3.7 | 186.0 ± 61.2 | |
| AZM+ | 6.4 ± 0.7 | 66.4 ± 10.3† | 44.8 ± 3.3 | 98.2 ± 30.5 | ||
| LPS 48 h | AZM- | 38.2 ± 4.3 | 232.9 ± 31.9 | 58.8 ± 8.2 | 155.6 ± 31.5 | |
| AZM+ | 26.7 ± 2.4* | 192.2 ± 27.0 | 15.4 ± 3.4‡ | 128.7 ± 21.8 | ||
| WT | LPS 3 h | AZM- | 5.0 ± 0.9 | 0.7 ± 0.1 | 17.7 ± 1.1 | 49.8 ± 20.6 |
| AZM+ | 4.4 ± 0.5 | 1.7 ± 0.9 | 21.4 ± 2.0 | 44.0 ± 6.7 | ||
| LPS 24 h | AZM- | 5.0 ± 1.7 | 93.3 ± 23.3 | 25.8 ± 1.6 | 55.0 ± 22.3 | |
| AZM+ | 7.1 ± 1.7 | 116.4 ± 22.7 | 31.2 ± 3.4 | 70.6 ± 18.6 | ||
| LPS 48 h | AZM- | 15.7 ± 4.4 | 142.3 ± 25.7 | 64.9 ± 8.6 | 168.2 ± 25.3 | |
| AZM+ | 14.1 ± 2.1 | 146.5 ± 17.0 | 54.9 ± 4.9 | 160.8 ± 19.1 | ||
Values are means ± SEM for 6–9 animals per each of the 12 subgroups. *p < 0.05; †p < 0.005; ‡p < 0.0005 for comparison of corresponding mean value vs. that obtained for the same parameter at the same time point without pre-treatment with azithromycin.