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. 2006 Nov 21;4(12):e409. doi: 10.1371/journal.pbio.0040409

Figure 5. GABAergic Inputs Evoked by ML Stimulation.

Figure 5

(A) Example of evoked IPSCs recorded from a pup-born DGC in response to extracellular stimulation of the ML before (control), during (BMI), and after (wash) application of 20 μM BMI. Scale bars indicate 25 ms, 20 pA. Inset: schematic diagram of the DG showing the position of the bipolar electrode for the stimulation of ML interneurons. Experiments were carried out with internal solution containing low [Cl−].

(B) Kinetics of IPSCs evoked on pup versus adult DGCs (pup, n = 6; adult, n = 14; rise time, p = 0.85; decay time, p = 0.90, paired t-tests). Inset: scaled averages of IPSCs (pup, green; adult, red). Scale bar indicates 50 ms.

(C) and (D) Examples of I-V curves of evoked IPSCs recorded from pup (C) or adult (D) DGCs. Top: sample traces of IPSCs recorded at the holding potentials shown on the right (mV). Scale bar indicates 50 ms, 10 pA. Bottom: I-V plots measured at the time indicated by the arrowheads.

(E) EGABA of IPSCs (pup, n = 7; adult, n = 11; p = 0.67, t-test).

(F) Slope conductance measured at the IPSC peak (n, same as in [E]; p = 0.23; t-test). All experiments conducted in the presence of kyn (4 mM) in neurons aged 12 wk (P7) and 7 wk (P42).