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. 1999 Apr 13;96(8):4575–4580. doi: 10.1073/pnas.96.8.4575

Figure 4.

Figure 4

Expression of a CYP19(I.1)−501:hGH fusion gene is detectable in transgenic mouse placenta as early as E10.5 and is developmentally regulated. Placentae at E10.5, E15.5, and E17.5 were obtained from a single line of transgenic mice carrying the CYP19(I.1)−501:hGH fusion gene. Total RNA was isolated and analyzed by Northern blotting for hGH expression by using a 32P-labeled hGH cDNA probe. To evaluate loading efficiency, the blot was stripped and reprobed with a 32P-labeled cyclophilin cDNA.