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. 1999 Apr 13;96(8):4575–4580. doi: 10.1073/pnas.96.8.4575

Figure 5.

Figure 5

Expression of a CYP19(I.1)−501:hGH fusion gene is restricted to labyrinthine trophoblast. Placental tissues obtained from E10.5 or E17.5 fetal mice carrying the CYP19(I.1)−501:hGH (A– C) transgene were processed for in situ hybridization by using a 35S-labeled antisense hGH cRNA probe and exposed to photographic emulsion for 1–2 weeks. Bright- and dark-field microscopy then were performed. (A) Dark-field micrograph of placental tissue section from an E10.5 transgenic mouse carrying the CYP19(I.1)−501:hGH fusion gene hybridized with radiolabeled hGH antisense cRNA probe. (B) Bright-field micrograph of the hematoxylin-stained E10.5 placental tissue section shown in A. (C) Dark-field micrograph of placental tissue section from an E17.5 transgenic mouse carrying the CYP19(I.1)−501:hGH fusion gene incubated with radiolabeled hGH antisense cRNA probe. (D) Dark-field micrograph of E17.5 placental section from a nontransgenic mouse incubated with radiolabeled hGH antisense cRNA probe. gc, trophoblast giant cell; sp, spongiotrophoblast; lab, labyrinthine trophoblast; ud, uterine decidual tissue.