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. 2006 Oct 19;103(44):16260–16265. doi: 10.1073/pnas.0607795103

Fig. 4.

Fig. 4.

PGC-1α suppresses FoxO3 action. (a Left) Plasmids bearing a luciferase reporter driven by the atrogin-1 promoter, c.a.FoxO3A, and PGC-1α were electroporated into the intact tibialis anterior muscles of adult mice, and luciferase activity was measured in extracts from the muscles 1 week later. (a Right) As in a Left, but using a canonical FoxO sequence (DAF16) driving the luciferase gene. (b) c.a.FoxO3 binding to a FoxO response element in the atrogin-1 promoter is blocked by PGC-1α. Muscles were collected 8 days after c.a.FoxO3 transfection with or without FLAG-PGC-1α, and ChIP assays were performed as described. (c) Constitutively active HA-tagged FoxO3A with or without FLAG-PGC-1α was electroporated into the intact tibialis anterior muscles of adult mice. Muscles were harvested after 8 days, serially sectioned, and subjected to immunohistochemistry (Left) or fiber size quantification (Right).