Fig. 2.

Cells expressing p53 stop making DNA in PALA, whereas those lacking p53 continue to cycle. (A) FACS analysis of SKOV3 and SKP53 cells treated with 250 μM PALA for 72 h. The cells were stained with propidium iodide to measure DNA content. Percentages of cells in G₁, G₂/M, and S are shown in each image. (B) Incorporation of BrdU. Untreated and treated SKOV3 and SKP53 cells were labeled with BrdU for 4 h, fixed in methanol, stained with anti-BrdU, and measured by FACS.