Fig. 5.

Treatment with MIC-1 protects p53-null SKOV3 cells from PALA. (A) Cells in 250 μM PALA were treated with various concentrations of MIC-1 (replenished daily), and after 12 days, the plates were stained with methylene blue and photographed. (B) Cells were treated with a 50 ng/ml concentration of either MIC-1 or TGF-β2 and 250 μM PALA. Twelve days later, the cells were photographed, and their viability was measured by trypan blue exclusion.