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. 2006 Oct 18;103(44):16290–16295. doi: 10.1073/pnas.0607856103

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© 2006 by The National Academy of Sciences of the USA

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Fig. 3. — Cdc55 regulates sister chromatid segregation independently of Pds1 in response to DNA damage. (A) Pds1 is stabilized in cdc13–1 Δcdc55 mutants. G₁-arrested cells of cdc13–1 PDS1-Myc18 and cdc13–1 Δcdc55 PDS1-Myc18 were released into YPD medium at 34°C. Crude protein extracts were prepared for Western blotting analysis. Cell-budding profile is shown in Right. (B) Mcd1 protein level is persistent in Δcdc55 mutants in the presence of DNA damage. G₁ cells of cdc13–1 MCD1-HA6 and cdc13–1 Δcdc55 MCD1-HA6 were released at 34°C and withdrawn to prepare protein extracts for immunoblotting of Mcd1-HA6. The budding index is shown in Right.