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Time-lapse phase contrast/fluorescence microscopy demonstrating the appearance of P41-YFP before cessation of gliding. Selected frames over a 24-min observation during growth in a chamber slide. Yellow arrowheads indicate the leading P41-YFP focus, and green arrowheads indicate the trailing P41-YFP focus in a single gliding cell. (Scale bar, 1 μm.) To see the trailing focus unpaired with P30-CFP, see Movie 4, which is published as supporting information on the PNAS web site.
