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Phase contrast/fluorescence microscopy of M. pneumoniae cells producing P30-CFP and P41-YFP. (Left) P30-CFP fluorescence images. (Center) P41-YFP fluorescence images. (Right) Merged phase contrast and P41-YFP plus P30-CFP fluorescence images. (A and B) Cells with paired P30-CFP and P41-YFP foci. The circle in B indicates faint and diffuse P30-CFP focus. (C and D) Cells with unpaired P41-YFP foci (arrows). (Scale bars, 1 μm.)
