Fig. 5.

Quantitation of P30-YFP fluorescence intensity over time. Fluorescence was measured in 12-min intervals for 10 cells that formed new P30-YFP foci during the observation period and 10 cells that did not. The average fluorescence for the latter group (asterisks on the dashed line, with positive standard deviations indicated) is compared with fluorescence of existing (open symbols) and new (solid symbols) P30-YFP foci in four individual, representative cells that began with a single fluorescent focus that was duplicated at approximately the same time point during the observation period and for which no collisions or other contact with neighboring cells was observed.