Fig. 5.

Density sedimentation of RG leakage reactions. (A) Reactions performed with or without RGs were fractionated on Percoll gradients. Fractions were analyzed for virus particles by immunoblot, and gels were stained with Coomassie after transfer to indicate the location of RGs in the gradient (bands labeled “RG”). (B and C) RG leakage reactions performed with [³H]myristate-labeled ISVPs were fractionated, and each fraction was analyzed for the presence of myristoylated μ1N by scintillation counting. Two separate experiments are shown in B and C. The location of RGs and virus particles in the gradient are indicated (C Bottom) by Coomassie staining and immunoblot, respectively. T, material recovered from reaction tube.