Figure 2.

Effect of MS-27-275 of p21^WAF1/CIP1 and gelsolin. (A) Accumulation time courses of mRNA and protein. Total RNA (10 μg) and lysate (45 μg of total protein) extracted from K562 cells exposed to 0.3 μM MS-27-275 or compound 2 for the indicated periods were used for Northern and Western blotting, respectively. (B) Western blot analysis of p53, p21^WAF1/CIP1, and pRb. Lysates (45 μg of total protein) of the cells exposed to 0.3 μM MS-27-275 for the indicated periods were examined by Western blot analysis by using antibodies specific to p53, p21^WAF1/CIP1, and pRb. The details are described in Materials and Methods.