Table 1.
Genes and ORFs in DNA regions associated with chloromethane utilization
| Gene orf | Length, aa | Calculated Mr, kDa | Gene
|
Inferred function | Sequence comparison of representative hit: % protein sequence identity* | Identity %† | |
|---|---|---|---|---|---|---|---|
| Begins | Ends | ||||||
| Cluster I | |||||||
| cobU | 342 | 34.9 | 1,502 | 474 | Cobalamin biosynthesis | CobU (P. denitrificans) (P29935) | 57 |
| folC | 467 | 49.8 | 2,942 | 1,539 | Folylglutamate synthetase | FolC (E. coli) (P08192) | 32 |
| folD | 306 | 32.4 | 3,865 | 2,945 | 5,10-methylene-H4folate dehydrogenase/5,10-methenylH4folate cyclohydrolase | FolD (E. coli) (P24186) | 49 |
| purU | 287 | 32.7 | 4,849 | 3,986 | 10-formyl-H4folate hydrolase | PurU (Corynebacterium sp.) (Q46339) | 47 |
| orf414 | 414 | 43.7 | 5,628 | 6,872 | Unknown | Orf (Mycobacterium tuberculosis) (P72042) | 31 (143 aa) |
| cmuA | 617 | 67.0 | 6,897 | 8,750 | Methyltransferase/ | MtbA (M. barkeri) (O30640) | 24/32‡ |
| corrinoid protein | MtmC (M. barkeri) (O30641) | ||||||
| Cluster II | |||||||
| cobQ | >424 | ND | <1 | 1,275 | Cobalamin biosynthesis | CobQ (P. denitrificans) (P29932) | 59 |
| cobD | 330 | 34.2 | 2,275 | 1,283 | Cobalamin biosynthesis | CobD (P. denitrificans) (P21634) | 55 |
| orf219 | 219 | 24.9 | 3,345 | 2,686 | Unknown | (Synechocystis sp.) (Q55963) | 32 (117 aa) |
| metF | 320 | 34.3 | 3,507 | 4,469 | 5,10-methylene-H4folate reductase | Orf (Saccharomyces cerevisiae) (P53128) | 24 (156 aa) |
| cmuB | 311 | 33.3 | 4,703 | 5,638 | Methyl transfer | MtrH (M. thermoautotrophicum) (P80187) | 30 |
| cmuC | 378 | 41.2 | 5,635 | 6,771 | Methyl transfer | MtaA (M. barkeri) (Q48949) | 28 (104 aa) |
| orf361 | 361 | 37.5 | 7,971 | 6,886 | Cobalamin biosynthesis | MTH808 (M. thermoautotrophicum) (O26899) | 35 |
| cobC | >162 | ND | >8,456 | 7,968 | Cobalamin biosynthesis | CobC (P. denitrificans) (P21633) | 38 |
ND, not determined.
*Accession numbers from SwissProt or Trembl databases shown in parentheses.
†
Sequence identity is over the entire length of the shorter of the two compared sequences, except where noted.
‡
MtbA sequence (339 aa) can be aligned to residues 7–353 of CmuA, and the MtmC sequence (217 aa) can be aligned to residues 401–607 of CmuA, respectively.