Figure 2.

Genomic organization of the human ADAR1 gene. (A) Organization of the exons and introns within the 5′ region of the ADAR1 gene. The 5′-most seven exons are indicated to scale by filled boxes numbered 1B to 6; introns and the 5′ flanking regions are indicated by the solid lines. P_I corresponds to the IFN-inducible promoter upstream of exon 1A. The entire gene spans approximately 39 kb in length and contains 16 exons including exon 1B. Shown to scale are the P1-phage genomic clone P1–249, the overlapping λ-phage genomic clone λ176, and the 5′-portion of the overlapping P1 clones P1–652 and P1–959. The restriction map shows cleavage sites for BamHI (B) and XhoI (X) spanning the ≈60-kb region upstream of exon 6. For the 5.8-kb BamHI fragment that includes exon 1B, cleavage sites for AvaI, HindIII, and XbaI are included; no PstI sites are present in the fragment. (B) Southern gel-blot of P1- and λ-phage genomic clones, either uncut or digested with BamHI, HindIII, or PstI as indicated. The probe was ³²P-labeled exon 1B.