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. 1999 Apr 13;96(8):4621–4626. doi: 10.1073/pnas.96.8.4621

Figure 5.

Figure 5

Identification of a constitutively active ADAR1 gene promoter ≈9-kb upstream of the interferon-inducible promoter. Genomic DNA restriction fragments derived from the 5.8-kb BamHI fragment (Fig. 2) that includes exon 1B were inserted into the promoter-less pCAT-Basic plasmid. Promoter activities observed in human U cells transfected with the indicated CAT reporter plasmids are shown as percent of the conversion of [14C]chloramphenicol to the acetylated derivatives. Open bars refer to cells left untreated, and hatched bars refer to cells treated with IFN-α. pCAT-Control, the CAT reporter gene linked to the simian virus 40 promoter and enhancer; pCAT-Basic, the promoter-less plasmid vector without inserted human genomic DNA; pCAT-0.6Pi (S/X), the IFN-inducible PI promoter present within the 591-bp Sac-Xho genomic DNA fragment inserted into the CAT-Basic plasmid (16).