Figure 1.

5′ regulatory region of the rat AChE gene. (Upper) Nucleotide sequence of the rat AChE promoter aligned with corresponding regions from the mouse and human genes. The first nucleotide in the initiator element (Inr; underlined) is designated as +1. Note that the sequence in the rectangular box (+5 to +75) exhibits more than 97% identity with exon 1 in the mouse and human AChE genes. Black arrows indicate EGR-1/Sp1 clusters; oval shows an AP2 site; and white arrows indicate E-boxes. (Lower) Schematic representation of the Inr and other consensus sites for DNA-binding proteins present in a larger promoter fragment as well as in the first intron of the gene.