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. 1999 Apr 13;96(8):4627–4632. doi: 10.1073/pnas.96.8.4627

Figure 3.

Figure 3

(A) Schematic representation of several AChE promoter–reporter gene constructs used for the in vivo studies. Note that the four N-boxes (N) found in GRAP are retained in NRAP, whereas only the first intronic N-box at position +755 bp is still present in FRAP (R indicates those that are in reverse orientation). Arrow points to the first nucleotide of the Inr. (B and C) Total number of events (B) and percentage of synaptic events (C) seen in TA muscles after injection of the different constructs. Note that the pattern of expression of the reporter gene is unchanged despite large deletions of the 5′ and 3′ regions in the original 5.3-kb fragment (compare GRAP with NRAP and FRAP). However, note that deletion of an additional 499-bp intronic region abolished AChE promoter activity (compare FRAP with RAP). Star indicates no expression. Mean ± SE is shown; a minimum of 10 muscles were analyzed per construct.