Table 1.
Effect of underexpression of βAPP on brain weight, corpus callosum defect frequencies, and forebrain commissure sizes in all five lines of transgenic mice
| Line | βAPPΔ/ΔB6 | βAPPΔ/ΔB6-Sv | βAPPΔ/ΔSv | βAPPΔ/ΔB6-Ola | βAPPΔ/ΔSv-Ola |
|---|---|---|---|---|---|
| Brain weight (g)178 | P < 0.0005 (n = 40) | P < 0.0030 (n = 68) | P < 0.0005 (n = 59) | P < 0.0030 (n = 37) | P < 0.0085 (n = 38) |
| Mutant (mean ± SE) | 0.415 ± 0.006 | 0.482 ± 0.006 | 0.436 ± 0.005 | 0.490 ± 0.007 | 0.478 ± 0.007 |
| Wild type (mean ± SE) | 0.455 ± 0.004 | 0.521 ± 0.009 | 0.490 ± 0.006 | 0.552 ± 0.016 | 0.519 ± 0.008 |
| Wild type - mutant† | 0.040 (9%) | 0.039 (8%) | 0.054 (12%) | 0.062 (12%) | 0.041 (8%) |
| Corpus callosum score‡ | ns(n = 40) | ns(n = 75) | P < 0.0005 (n = 108) | ns(n = 37) | P < 0.0015 (n = 37) |
| Mutant | n = 19 | n = 45 | n = 66 | n = 24 | n = 17 |
| Normal | 19 (100%) | 43 (95.6%) | 1 (1.5%) | 23 (95.8%) | 0 (0%) |
| Dyscallosal | 0 (0%) | 1 (2.2%) | 4 (6.1%) | 1 (4.2%) | 2 (11.1%) |
| Acallosal | 0 (0%) | 1 (2.2%) | 61 (92.4%) | 0 (0%) | 16 (88.9%) |
| Wild type | n = 21 | n = 30 | n = 42 | n = 13 | n = 20 |
| Normal | 21 (100%) | 30 (100%) | 28 (66.7%) | 13 (100%) | 6 (30.0%) |
| Dyscallosal | 0 (0%) | 0 (0%) | 8 (19%) | 0 (0%) | 9 (45.0%) |
| Acallosal | 0 (0%) | 0 (0%) | 6 (14.3%) | 0 (0%) | 5 (25.0%) |
| Corpus callosum, mm2178 | P < 0.0148 (n = 39) | P < 0.0472 (n = 58) | P < 0.0004 (n = 50) | (n = 10) | P < 0.0004 (n = 38) |
| Mutant (mean ± SE) | 1.090 ± 0.018 | 1.151 ± 0.034 | 0.000 ± 0.000 | 1.030 ± 0.104 | 0.043 ± 0.040 |
| Wild type (mean ± SE) | 1.171 ± 0.020 | 1.310 ± 0.045 | 0.678 ± 0.119 | — | 0.438 ± 0.930 |
| Wild type - mutant† | 0.081 (7%) | 0.159 (13%) | 0.678 (200%) | — | 0.395 (164%) |
| Adjusted to b.w. 0.476g:§ | |||||
| Mutant (mean ± SE) | 1.141 ± 0.018 | 1.144 ± 0.036 | 0.000 ± 0.000 | 1.034 ± 0.102 | 0.043 ± 0.036 |
| Wild type (mean ± SE) | 1.188 ± 0.020 | 1.270 ± 0.045 | 0.665 ± 0.117 | — | 0.413 ± 0.092 |
| Wild type - mutant† | 0.047 (4%) | 0.126 (10%) | 0.665 (200%) | — | 0.370 (162%) |
| Hipp. commissure, mm2178 | P < 0.0004 (n = 39) | P < 0.0064 (n = 58) | P < 0.0004 (n = 50) | (n = 10) | P < 0.0004 (n = 38) |
| Mutant (mean ± SE) | 0.259 ± 0.006 | 0.296 ± 0.007 | 0.121 ± 0.010 | 0.296 ± 0.015 | 0.133 ± 0.018 |
| Wild type (mean ± SE) | 0.304 ± 0.007 | 0.337 ± 0.008 | 0.291 ± 0.016 | — | 0.279 ± 0.016 |
| Wild type - mutant† | 0.045 (16%) | 0.041 (13%) | 0.170 (83%) | — | 0.146 (71%) |
| Adjusted to b.w. 0.476g:§ | |||||
| Mutant (mean ± SE) | 0.287 ± 0.004 | 0.292 ± 0.008 | 0.171 ± 0.010 | 0.297 ± 0.014 | 0.133 ± 0.018 |
| Wild type (mean ± SE) | 0.314 ± 0.006 | 0.317 ± 0.008 | 0.284 ± 0.015 | — | 0.257 ± 0.015 |
| Wild type - mutant† | 0.027 (9%) | 0.025 (8%) | 0.113 (50%) | — | 0.124 (64%) |
| Ant. commissure, mm2178 | P < 0.0080 (n = 39) | ns(n = 57) | P < 0.0020 (n = 50) | (n = 10) | P < 0.0008 (n = 38) |
| Mutant (mean ± SE) | 0.118 ± 0.003 | 0.134 ± 0.004 | 0.096 ± 0.002 | 0.123 ± 0.007 | 0.100 ± 0.003 |
| Wild type (mean ± SE) | 0.132 ± 0.003 | 0.144 ± 0.004 | 0.119 ± 0.005 | — | 0.116 ± 0.003 |
| Wild type - mutant† | 0.014 (11%) | 0.010 (7%) | 0.023 (21%) | — | 0.016 (15%) |
| Adjusted to b.w. 0.476g:§ | |||||
| Mutant (mean ± SE) | 0.126 ± 0.002 | 0.134 ± 0.004 | 0.100 ± 0.002 | 0.124 ± 0.006 | 0.099 ± 0.003 |
| Wild type (mean ± SE) | 0.134 ± 0.003 | 0.139 ± 0.004 | 0.119 ± 0.005 | — | 0.112 ± 0.003 |
| Wild type - mutant† | 0.008 (6%) | 0.005 (4%) | 0.019 (17%) | — | 0.013 (12%) |
178
Mann–Whitney U with Bonferroni correction for multiple comparisons.
†
Percentual differences are shown in parentheses and were computed by using the formula 200 (Xwild−Xmut)/(Xwild+Xmut).
‡
χ2 with Bonferroni correction for multiple comparisons.
§
Commissure sizes were adjusted to an average brain weight of 0.476 g by using linear regression. For details see Materials and Methods.