Figure 6.

15d-PGJ₂ does not inhibit nuclear translocation or DNA-binding ability of NFκB. (A) Nuclear extracts were prepared from untreated BV-2 cells (NT) or BV-2 cells treated with the control buffer (1), 80 ng/ml of LPS (2), LPS + 1 μM 15d-PGJ₂ (3), or 15d-PGJ₂ alone (4) for 1 and 3 h. p65 protein levels were determined by Western blotting. (B) The same nuclear extracts as in A were analyzed for the presence of NFκB-binding activity in a gel-shift assay. Arrow indicates the position of the shifted band. Experiments were repeated three (A) and two (B) times with similar results, and data from one representative of each experiment are shown.