Figure 2.

Nuclear translocation of MAPK in response to E2. Indirect immunofluorescence staining of MCF-7 cells using primary antibodies anti-Erk1/2 (Upper Left) and anti-active-MAPK antibodies (Upper Right and Lower). Cells either were left untreated (Upper) or treated with EGF (Lower Left) and estrogen (Lower Right) for 30 min before incubation with FITC secondary antibody.